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glyceraldehyde 3 phosphate dehydrogenase  (Proteintech)


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    Structured Review

    Proteintech glyceraldehyde 3 phosphate dehydrogenase
    Glyceraldehyde 3 Phosphate Dehydrogenase, supplied by Proteintech, used in various techniques. Bioz Stars score: 98/100, based on 10658 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+gapdh/GAPDH+Monoclonal+antibody/pmc13651863-280-6-27
    Average 98 stars, based on 10658 article reviews
    glyceraldehyde 3 phosphate dehydrogenase - by Bioz Stars, 2026-10
    98/100 stars

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    Related Articles

    MTT Assay:

    Article Title: Prognostic and immunotherapeutic efficacy predictive model for gastric cancer based on ferroptosis- and autophagy-related genes.
    Article Snippet: .. The reagents and biological materials used in this study are as follows: cell lines such as GES‐1 (human normal gastric mucosal epithelial cell lines), AGS, HGC27, and MKN7 (Cell Bank of Type Culture Collection of the Chinese Academy of Sciences); antibodies such as anti‐ALDH3A2 (Abcam, A19038), β‐Actin, anti‐GAPDH (Proteintech, Cat No: 10494–1‐AP), anti‐E‐cadherin (Proteintech, Cat No: 60335 1‐Ig), anti‐N‐cadherin (Proteintech, Cat No: 220–1‐AP), anti‐Vimentin (Proteintech, Cat No: 10366‐1‐AP), and HRP Goat Anti Rabbit IgG (H+L) (Abclonal, AS014); and chemicals such as crystal violet (Aladdin, C110703), 3‐(4,5‐dimethylthiazolyl‐2)‐2,5‐diphenyltetrazolium bromide (MTT, Aladdin: M158055), and BeyoClick EdU‐488 Cell Proliferation Detection Kit (Beyotime, C0071S). .. Establishing stably transfected cell lines The lentivirus (vector: GV492) used in this study was supplied by Jikai Gene (Shanghai, China).

    CCK-8 Assay:

    Article Title: Direct targeting of LYN kinase by fluphenazine triggers ferroptosis to suppress lung adenocarcinoma.
    Article Snippet: Department of Pulmonary and Critical Care Medicine, The First Affiliated Hospital of Soochow University, Suzhou, China Department of Pharmacy, The First Affiliated Hospital of Soochow University, Suzhou, China College of Pharmaceutical Sciences, Soochow University, Suzhou, China Institute of Respiratory Diseases, Soochow University, Suzhou, China Suzhou Key Laboratory for Respiratory Diseases, Suzhou, China Cancer Institute, Suzhou Medical College, Soochow University, Suzhou, China

    Cell-Signaling:

    Article Title: Direct targeting of LYN kinase by fluphenazine triggers ferroptosis to suppress lung adenocarcinoma.
    Article Snippet: Department of Pulmonary and Critical Care Medicine, The First Affiliated Hospital of Soochow University, Suzhou, China Department of Pharmacy, The First Affiliated Hospital of Soochow University, Suzhou, China College of Pharmaceutical Sciences, Soochow University, Suzhou, China Institute of Respiratory Diseases, Soochow University, Suzhou, China Suzhou Key Laboratory for Respiratory Diseases, Suzhou, China Cancer Institute, Suzhou Medical College, Soochow University, Suzhou, China

    Article Title: Kuwanon E sensitizes hepatocellular carcinoma cells to disulfidptosis via MTOR-mediated autophagy blockade.
    Article Snippet: Disulfidptosis, a recently identified form of regulated cell death driven by toxic intracellular disulfide accumulation and subsequent collapse of the actin cytoskeleton, represents a promising therapeutic vulnerability in hepatocellular carcinoma (HCC), particularly for tumors exhibiting metabolic reprogramming and resistance to conventional apoptosis-based therapies.. In this study, we screened eleven representative bioactive constituents derived from Cortex Mori, including stilbenoids, flavonoids, coumarins, and phenolic acids, under glucose deprivation conditions that trigger disulfidptosis, and identified Kuwanon E (KE) as the most potent sensitizer of disulfidptosis in HCC cells.. Integrative network pharmacology, RNA sequencing, and untargeted metabolomics revealed that KE preferentially targets the MTOR signaling pathway and autophagic flux.

    Article Title: Validation of CRISPRoff guide RNAs for stable repression of human AKT1
    Article Snippet: .. Equal amounts (20 μg) of total protein were subjected to SDS-PAGE and immunoblotting using anti-Akt1 (C73H10, #2938; Cell Signaling Technology) and anti-GAPDH (#60004-1-Ig; Proteintech) antibodies. .. After incubation with HRP-conjugated secondary antibodies, immunoreactive bands were visualized using Chemi-Lumi One Super (Nacalai Tesque, Kyoto, Japan) and detected with a Luminograph III imaging system (ATTO, Tokyo, Japan).

    Article Title: Investigating the mechanism of Xiongfu formula and its active ingredient Z-Ligustilide alleviates chronic migraine by modulating the MAPK/ERK/CREB pathway and microglia-mediated neuroinflammation.
    Article Snippet: Ethnopharmacological relevance: Chronic migraine is a recurrent throbbing headache, which is often attributed to stagnated Qi and impaired blood circulation from the perspective of traditional Chinese medicine.. Xiongfu formula (CRCR), consisting of Chuanxiong Rhizoma and Cyperi Rhizoma, is a classic herbal formula in treating migraine from the treatise Jingyue Quanshu during the Ming Dynasty.. Aim of the study: This study sought to look at the potential molecular mechanism of CRCR and its active constituents for chronic migraine therapy.

    SDS Page:

    Article Title: Validation of CRISPRoff guide RNAs for stable repression of human AKT1
    Article Snippet: .. Equal amounts (20 μg) of total protein were subjected to SDS-PAGE and immunoblotting using anti-Akt1 (C73H10, #2938; Cell Signaling Technology) and anti-GAPDH (#60004-1-Ig; Proteintech) antibodies. .. After incubation with HRP-conjugated secondary antibodies, immunoreactive bands were visualized using Chemi-Lumi One Super (Nacalai Tesque, Kyoto, Japan) and detected with a Luminograph III imaging system (ATTO, Tokyo, Japan).

    Western Blot:

    Article Title: Validation of CRISPRoff guide RNAs for stable repression of human AKT1
    Article Snippet: .. Equal amounts (20 μg) of total protein were subjected to SDS-PAGE and immunoblotting using anti-Akt1 (C73H10, #2938; Cell Signaling Technology) and anti-GAPDH (#60004-1-Ig; Proteintech) antibodies. .. After incubation with HRP-conjugated secondary antibodies, immunoreactive bands were visualized using Chemi-Lumi One Super (Nacalai Tesque, Kyoto, Japan) and detected with a Luminograph III imaging system (ATTO, Tokyo, Japan).

    Article Title: A novel tRNA-derived fragment, tRF-16-WSNKP9D promotes malignant progression of colorectal carcinoma through mediating PFN1.
    Article Snippet: .. Immunoblotting procedures incorporated a specific panel of primary antibodies, including anti-GAPDH (60004-1- Ig), anti-PFN1 (11680-1-AP), anti-BAX (50599-2-Ig), anti-BCL-2 (12789-1-AP), and anti-cleaved caspase 3 (68773-1-Ig), all sourced from Proteintech (Shanghai, China). .. Additionally, downstream molecular interactions were interrogated utilizing the Pierce Magnetic RNA-Protein Pull-Down system (Thermo Fisher Scientific).

    Binding Assay:

    Article Title: Investigating the mechanism of Xiongfu formula and its active ingredient Z-Ligustilide alleviates chronic migraine by modulating the MAPK/ERK/CREB pathway and microglia-mediated neuroinflammation.
    Article Snippet: Ethnopharmacological relevance: Chronic migraine is a recurrent throbbing headache, which is often attributed to stagnated Qi and impaired blood circulation from the perspective of traditional Chinese medicine.. Xiongfu formula (CRCR), consisting of Chuanxiong Rhizoma and Cyperi Rhizoma, is a classic herbal formula in treating migraine from the treatise Jingyue Quanshu during the Ming Dynasty.. Aim of the study: This study sought to look at the potential molecular mechanism of CRCR and its active constituents for chronic migraine therapy.

    Incubation:

    Article Title: Investigating the mechanism of Xiongfu formula and its active ingredient Z-Ligustilide alleviates chronic migraine by modulating the MAPK/ERK/CREB pathway and microglia-mediated neuroinflammation.
    Article Snippet: Ethnopharmacological relevance: Chronic migraine is a recurrent throbbing headache, which is often attributed to stagnated Qi and impaired blood circulation from the perspective of traditional Chinese medicine.. Xiongfu formula (CRCR), consisting of Chuanxiong Rhizoma and Cyperi Rhizoma, is a classic herbal formula in treating migraine from the treatise Jingyue Quanshu during the Ming Dynasty.. Aim of the study: This study sought to look at the potential molecular mechanism of CRCR and its active constituents for chronic migraine therapy.

    Article Title: Doxorubicin-induced cardiotoxicity: SESN2-directed FBXO7 ubiquitination of CRY1 restores glycolytic homeostasis.
    Article Snippet: .. Membranes were blocked and incubated overnight at 4°C with the 205 following primary antibodies: anti-SESN2 (1:2000; Cat# 10795-1-AP, Proteintech, 206 China), anti-CRY1 (1:4000; Cat# 13474-1-AP, Proteintech, China),anti-FBXO7(1:100; 207 Cat# sc-271763, Santa Cruz Biotechnology, USA), anti-β-ACTIN (1:10000; Cat# 208 66009-1-Ig, Proteintech, China) or anti-GAPDH(1:10000; Cat# 60004-1-Ig, Proteintech, 209 China). .. 210 After washing, membranes were incubated for 1 h at room temperature with the 211 following HRP-conjugated secondary antibodies: goat anti-rabbit IgG (1:8000; Cat# 212 111-035-003, Jackson ImmunoResearch, USA) or goat anti-mouse IgG (1:8000; Cat# 213 115-035-003, Jackson ImmunoResearch, USA).The signal intensities were visualized 214 using an Enhanced chemiluminescence (ECL) detection kit (Cat# BL520B, Biosharp, 215 China) and quantified with ImageJ software.



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